By Saul Neidleman
"Advances in utilized Microbiology" covers a huge diversity of themes within the fields of utilized microbiology and biotechnology. those volumes supply articles which will be of curiosity to biotechnology researchers in academia and undefined, fermentation microbiologists, microbial ecologists, biochemical engineers and utilized microbiologists in different speciality parts.
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Additional info for Advances in Applied Microbiology, Vol. 38
Remove the flasks in the sequence in which they were placed in the bath, add 1ml of the CaCl, solution and 4 ml of the NaOH solution to each flask, swirl, and filter the suspension through a folded, 100-mm disk of Whatman No. 2 filter paper in a 65-mm (top diameter) short-stem funnel with a fluted bowl. Controls are analyzed in a similar manner, except that the PNP substrate is added after the CaCl, and NaOH solutions. 0 ml of MUB at the appropriate pH to a 50-ml Erlenmeyer flask, as described above.
Flagellates, ciliates, amoebas) separately. 1-ml aliquots from the test tubes under lowpower magnification ( x 100) for the presence of protozoa. , Cochran, 1950), as in the plate counts. The counts obtained by the solid and liquid media methods are similar, as indicated in Table 11. 28 G. STOTZKY ET AL. TABLE I1 COMPARISON OF NUMBERS OF PROTOZOA ENUMEMTED ON SOLID OR IN GEM or homologous host added to soil Control (Hz0) E. coli W3110 E . 59 Osee text for details. 11. Physiological Groups The effects of GEMS on the diversity of the soil microbiota can also be estimated by measuring changes in physiological (nutritional) groups as an indicator of changes in bacterial populations and community structure.
Spread over each island a loopful of a suspension of mixed bacteria to serve as a food source for the protozoa. , 10-7 to the center of lo-, five replicate islands. Incubate the plates, without inverting, in the dark for 5 days at 24 & 2°C. Examine each island under low-power magnification [ x 100) for the presence of protozoa, both vegetative and encysted forms. Clearing of the inoculated biomass is presumptive for the presence of protozoa, and visual observation of protozoa (flagellates, ciliates, and amoebas) is used to confirm their presence.